Fig. 3. Effect of hypoxia on NSCLC cells. A549 and SPCA1 cells were cultured under hypoxia for 0, 12, 24 and 48h, and the gene and protein expressions were analyzed using RT-PCR and western blot assay, respectively. (A, B) NEAT1 expressions in A549 and SPCA1 cells. (C, D) Protein expressions of HIF-2α, E-cadherin, ZO-1, N-cadherin, and Vimentin in A549 and SPCA1 cells. (E, F) The migration and invasion of A549 and SPCA1 cells. *P<0.05, **P<0.01.